Obtaining and Use of the Recombinant Bovine Pregnancy-Associated Glycoprotein 1

Anara Ryskeldina, Indira Iskakova, Nurgul Sarina, Alexandr Shevtsov, Laura Syzdykova, Alexander Shustov, Yerlan M. Ramankulov, Marat Kuibagarov

2022

Full text Open access: Gold Metadata (CSL-JSON)

DOI 10.17582/journal.aavs/2022/10.10.2148.2159

Data confidence 70 medium

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Abstract

A convenient biochemical marker of cow pregnancy is the bovine pregnancy-associated glycoprotein 1 (boPAG1) antigen.The aim of this work was to produce a recombinant boPAG1 antigen and obtain monoclonal antibodies (mAbs) against boPAG1.We have obtained the boPAG1 cDNA and are reporting its nucleotide sequence.Bacterial expression of a portion of the natural gene encoding a mature form of boPAG1 failed.But a fusion protein made up of E. coli thioredoxin and boPAG1 was efficiently expressed in E. coli.Using the recombinant protein as an antigen, mAbs were generated.One mAb reacted with a protein present in extracts from cow placenta.The described results are useful for creating immunological reagents to detect pregnancy in cows, which is of great practical importance.