Аңдатпа
A convenient biochemical marker of cow pregnancy is the bovine pregnancy-associated glycoprotein 1 (boPAG1) antigen.The aim of this work was to produce a recombinant boPAG1 antigen and obtain monoclonal antibodies (mAbs) against boPAG1.We have obtained the boPAG1 cDNA and are reporting its nucleotide sequence.Bacterial expression of a portion of the natural gene encoding a mature form of boPAG1 failed.But a fusion protein made up of E. coli thioredoxin and boPAG1 was efficiently expressed in E. coli.Using the recombinant protein as an antigen, mAbs were generated.One mAb reacted with a protein present in extracts from cow placenta.The described results are useful for creating immunological reagents to detect pregnancy in cows, which is of great practical importance.