HAT-RAPD profiling of seven fungi isolated from central Kazakhstan soils

Konstantin Li, Assel Moldagulova, Eleonora Bekenova, Abzal Kazhibaev, Markhabbat Kairova, Maira S. Urazova, Serik Shaikhin, K Kh Almagambetov

2014

Closed access Metadata (CSL-JSON)

DOI 10.38150/sajeb.4(1).p17-23

Data confidence 70 medium

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Abstract

Stable HAT-RAPD (high annealing temperature) profiles were obtained for 7 micromycetes isolated from the central Kazakhstan soils with single non-specific primers. An arbitrary primer (decamer AP3), a primer derived from the intergenic spacer regions (T3B) and microsatellite primer (GTG)5 were applied in PCR. Precise and reproducible profiles of the amplification prod-ucts were obtained for all seven hyphomycetes isolates in spite of increased annealing temperature application. The number and size of amplicons reflect a species specificity. In some cases intraspecific variability was observed. Cluster analysis of the profiles demonstrated the phylogenetic relationships between isolates that are consistent with those of ITS-region genotyping. Obtained results completely justify the application of the HAT-RAPD in mark-ing and rapid identification of soil hyphomycetes.