The Aging‐related Approach to Detection of Mitotic Factor in Thoracic Duct Lymph of Rabbits

Alexander V. Kuznetsov, Ydyrys A. Almabayev, Ildar R. Fackhradiev, Aigul Y. Aigul, Aigul Almabayeva, Jandos J. Serikpayev, Baimakhan Tanabayev

2017

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DOI 10.20530/ijimhs425
Data confidence 70 medium
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Abstract

Introduction: It is known that mitosis is a fundamental process and it has been researched in normal as well as in pathology.It is suggested that the cause of a colossal number of research about mitosis is an empirical approach to the problem.For example, finding the optimal combination (MF) of only nine components (cytokines or any proteins) requires 181440 steps.Aim: In the study, the aging-related approach to detection of an explicit of mitotic factor MF to control mitosis through only one step is offered. Material and Methods:The following formula of MF has been invented: MF = ∆ (A-B).The formula includes: (a) Composition of the known components of thoracic duct lymph (TDL) of immature rabbits and (b) composition of the known components of mature rabbits TDL, ∆ -the difference of the components composition between "A" and "B." Results and Discussion: The approach should be carried out in the following succession: (1) To obtain samples of TDL of immature rabbits and TDL of mature rabbits (groups 1-"A" and 2-"B" accordingly) by special glass micropipette (Patent № 1495076, Russia), (2) to make lymph plasma from the samples of TDL of rabbits of group 1 and group 2, (3) to find a qualitative and quantitative components composition of lymph plasma and TDL cells of group 1 and group 2, and (4) to find the difference (MF/∆) between the lymph plasma of the components composition of groups 1 and 2. Consequently, the difference (∆) will be the sought-for MF.To study MF proteomic analysis can be used. Conclusions:The aging-related approach can be used to study different features of TDL, particularly cells division, identification of lymph MF, that can play a key role in access to cell dividing mechanism, and it is the way to elaborate new cancer drugs.