Abstract
The study of the phytochemical composition of plants of the Artemisia genus represents a relevant direction in modern pharmaceutical science due to their broad biological activity. The present research focuses on the analysis of the chemical components of the aerial parts of Artemisia rutifolia. The aim of this work was to isolate, identify, and characterize the major biologically active compounds from the ethanolic extract of the plant using advanced analytical methods. The research scope included extraction, fractionation, and spectroscopic identification of the isolated substances. Scientific relevance lies in expanding the knowledge of the chemical profile of Artemisia rutifolia extract, thus creating prospects for the development of novel pharmaceuticals and dietary supplements. Practical significance consists in the isolation of biologically active compounds with potential pharmacological activity. Methodology involved obtaining an ethanolic extract, subsequent liquid–liquid fractionation using petroleum ether and chloroform, and purification on a Sephadex LH-20 column. The structures of the isolated compounds were elucidated using NMR spectroscopy and comparison with authentic reference standards. Three compounds - monoterpene camphor, flavonol casticin, and sterol glycoside daucosterin - were isolated and identified from the Artemisia rutifolia extract, with their chemical structures and high purity confirmed by spectroscopic analysis. The contribution of this study lies in the detailed characterization of the components of a promising medicinal plant, thereby enriching pharmacognostic data on the genus Artemisia. The findings may serve as a basis for further pharmacological investigations and the development of novel therapeutic and dietary products.