The role of phosphorus in the biosynthesis of Penicillium cyclopium 2-11 pectinase enzymes

2019

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DOI 10.32523/2616-7034-2019-128-3-42-47
Data confidence 70 medium
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Abstract

A significant influence on the formation of pectinase enzymes has a source of nitrogen nutrition. The most effective, providing high synthesis of pectinase enzymes, are inorganic nitrogen compounds and primarily physiologically acidic nitrogen sources. We have found that for the active synthesis of P. cyclopium 2-11 pectinase enzymes, it is necessary to add more phosphorus to the medium than recommended by the апapek medium. When using trisubstituted ammonium phosphate as an nitrogen source, an additional amount of phosphorus is added to the medium along with it (0.164 g according to P). This determined the advantage of this nitrogen source over others. Therefore, when using other nitrogen sources as a source of nitrogen nutrition, it is necessary to take into account that in order to maximize the biosynthesis of P. cyclopium 2-11 pectinase enzymes, phosphorus should be added in an amount of 0.368 g / 100 ml of medium, which increases the biosynthesis of the PL enzymes in the immobilized culture in 10-11 time.