The optimal technological parameters of cultivation of the cell line VNK-21

И. А. Пунтус, K.N. Zhaslanova, G.M. Salkhozhayeva, Марат Тыныкулов, K.M. Urazov

2020

Full text Open access: Gold Metadata (CSL-JSON)

DOI 10.32523/2616-7034-2020-130-1-37-46

Data confidence 70 medium

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Abstract

The article presents the results of cultivation of cell lines VNK-21 and VNK-21(сl-13) stationary, roller, rollermonolayer-suspension methods. Optimal technological parameters of cell cultivation are given and growth modified enrichednutrient media are selected for the tested cell lines. Aspects of scaling the process of cell culture are considered.The optimal incubation conditions of the VNK-21(сl-13)line were selected: constant temperature +37±0.10C in the thermalroom, roller rotation speed 0.5-0.8 rpm, modified enriched nutrient medium FGM-C+DMEM (3:1), sieving multiplicity 1:15-1:25or sowing concentration 85-150 thousand CL/ml, cell removal method - versin+trypsin (9:1). A roller monolayer-suspensioncultivation method is proposed for accumulation of biomass of VNK-21(сl-13)cells in rollers.