Abstract
Relevance: Gastric cancer all over the world is ranked 4th in morbidity and 2nd in mortality [1], which is one of the most important social problems of society. GC is a complex disease that causes factors of environmental and host factors, causing factors that contribute to high mortality of gastric cancer, including its silent course, late clinical manifestations and underlying biological and genetic heterogeneity. Given the tacit and aggressive nature of gastric cancer, patients seek medical help in advanced stages. Modern science, having the opportunity to study methods for the study of oncological pathology, requires the search for diagnostic methods and the introduction of new personalized methods and monitoring in the treatment of oncological diseases. Phosphorylation of histone H2AX on Serine IY residues with the endpoint Carboxyl (which produces yH2AX) is a sensitive marker for DNA double-strand break (DSB) repair. Double-strand DNA breaks cause severe damage that can cause genomic instability, resulting in cancer [2,3] [4] Diseases of a person with defects in these processes often exhibit a predisposition to cancer [5]. A key component in DNA repair is the histone H2AX protein, which rapidly becomes phosphorylated at Serine IY residues from the carboxyl endpoint (Carboxyl endpoint) (Serina c-IY) in order to form yH2AX at the appropriate sites of the DB. Within 30 minutes after DB formation, a large number of yH2AX molecules form in chromatin around the site of decomposition, forming a focus where proteins involved in DNA repair and accumulation of chromatin remodeling are accumulated [6] This Amplification enables to detect individual DB with an anti-yH2AX antibody.