Pigment mutants of the green microalga Chlamydomonas reinhardtii: morphological properties and photosynthetic performance

Б. К. Заядан, Dmitry N. Matorin, Nurziya R. Akmukhanova, Asemgul K. Sadvakasova, Kenzhegul Bolatkhan, Ж Бауенова, Makpal M. Torekhanova

2019

Full text Open access: Gold Metadata (CSL-JSON)

DOI 10.26577/eje.2019.v59.i2.09

Data confidence 70 medium

How this score is calculated

It is built from checkable properties of the record itself: whether the article has a DOI, how many independent sources confirmed its data, whether the required fields are filled in, and how recent the latest confirmation is. Disagreement between sources and a missing identifier lower it. A value of 80 or above means the record was published without further checking; 60 to 79 means it is published at reduced confidence, with a manual check advisable. A record verified by an editor by hand is never pushed below 90 by a recalculation. This scores our data about the article, not the article itself.

Abstract

Pigment mutants CC-124y-1, СС-124у-2, and СС-124р-2 of the green microalga Chlamydomonasreinhardtii Dangeard were obtained by means of UV-induced mutagenesis. Morphological propertiesand photosynthetic activity of these mutants were examined. The mutants displayed a lowered contentof chlorophyll b and carotenoids. Analysis of fluorescence induction curves revealed the decreased ratesof electron transport in photosystem II (PSII), the increase in the fraction of QB-nonreducing centers, andthe enhancement of nonphotochemical fluorescence quenching. The mutations had no direct impact onoxidation of pigment P700 in PSI and on the decline of delayed fluorescence. The parameters of induction curves of prompt and delayed fluorescence are proposed for use in early diagnostics of UV-inducedmutagenesis. The pigment mutants obtained can be applied in biomonitoring studies.Key words: Chlamydomonas reinhardtii, chlorophyll a fluorescence, delayed fluorescence, JIP test,photosynthesis.