Abstract
O pisthorchis felineus (Rivolta, 1884) is the main liver fluke of the family Opisthorchiidae (Looss, 1899), which infects the liver of mammals and humans.O. felineus has been documented in humans and animals in 13 countries of the European Union (Pozio et al., 2013).This liver fluke also causes public health problems in the Russian Federation, Ukraine, Belarus and Kazakhstan (WHO, 1995).In Kazakhstan, this is the most common fluke species and is endemic in Pavlodar, Akmola, Karaganda, Northern Kazakhstan, Eastern Kazakhstan and Aktobe oblasts (Sultanov et al., 2014).O. felineus is considered responsible for serious diseases with high risks of cholangiocarcinoma emergence (IARC, 1994; Sripa et al., 2011; Armignacco et al., 2013; Chai et al., 2014; Fedorova et al., 2016).Early accurate diagnoses can prevent future problems and avoid health consequences.Various techniques have been developed for identifying opisthorchiasis agents for accurate diagnoses.One such method, coproovoscopy, is laborious, time-consuming, requires qualified specialists, and fails to accurately identify opisthorchiid eggs.Serological test studies have suggested using antigens or antibodies specific to O. felineus for the diagnosis of opisthorchiasis (Teimoori et al., 2016; Bulashev et al., 2016; Waikagul et al., 2002; Starkova et al., 2011; Nöckler et al., 2003; Klebanovskaia, 1981 Klebanovskaia, , 1985)).Various components of parasites are used as antigens for immunodiagnostics and quantitative analyses of immune responses in infected hosts.These antigens can be classified as excretory-secretory, somatic and egg antigens (Sun et al., 1969; Kotelkin et al., 1997).Antigens of Opisthorchis have been suggested as target proteins for the serodiagnosis of opisthorchiasis.Opisthorchis antigens prepared from adult worms (somatic antigens) have molecular weights of 105, 90, 89, 63, 22.8, 16 and 15 kDa and can be used for immunodiagnoses (Wongratanacheewin et al., 2003; Glupov et al., 1997; Borovikov et al., 2010; Billings et