Testing the process of accumulation of the virus sheep pox

K.N. Zhaslanova, G.M. Salkhozhayeva, Ж.А. Рахимжанова, Марат Тыныкулов, И. А. Пунтус, K.M. Urazov

2019

Full text Open access: Gold Metadata (CSL-JSON)

DOI 10.32523/2616-7034-2019-126-1-37-45

Data confidence 70 medium

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Abstract

The article presents the results of adaptation and development of optimal parameters of accumulation of sheep pox virus strain on the transplanted cell cultures 3-KG, YADK-04 and PO-2. Infectious activity in case of infection with VSP on a complete formed monolayer was 5.0-5.75 lg TCD 50 /cm 3 on the YADK line and 5.5-6.0 lg TCD 50 /cm 3 on 3-KG, with a plurality of infection 0.1-0.5 TCD 50 /CL., incubation time was 72-96 hours. The method of determining the infectious titer of the virus by test-tube and microplate methods is worked out, the correlation analysis is carried out. Analysis of the results of determining the infectious activity of the smallpox virus showed comparability of the data with a discrepancy of + 0,1-0,5 lg (+0,25) towards the microplate method (n=7), which indicates its higher sensitivity and accuracy.