Histological and Histochemical Methods for Staining of Insulin: A Comparative Analysis

G.G. Meyramov

2019

Full text Open access: Gold Metadata (CSL-JSON)

DOI 10.26717/bjstr.2019.13.002400

Data confidence 70 medium

How this score is calculated

It is built from checkable properties of the record itself: whether the article has a DOI, how many independent sources confirmed its data, whether the required fields are filled in, and how recent the latest confirmation is. Disagreement between sources and a missing identifier lower it. A value of 80 or above means the record was published without further checking; 60 to 79 means it is published at reduced confidence, with a manual check advisable. A record verified by an editor by hand is never pushed below 90 by a recalculation. This scores our data about the article, not the article itself.

Abstract

Various histochemical and histological methods are used for analysis of the state of histostructure and of insulin content in pancreatic B-cells.Aim of work: a comparative analysis of the results of using of 3 staining technologies refined and detailed by us: Aldehyde fucshine, AF, fluorescent diethylpseudoisocyanine method, PS and Dimethylnaphtylmetan, V4 (Victoria 4R, color index 42563).Intensity of fluorescence of A-chair of insulin (PS) measured using of histofluorimetric complex [1].Calculation of parameter FL= IF1/IF2 where IF2-intensity of fluorescence of intact B-cells (as 1.00) and IF1-B-cells in diabetes.Density of color (AF and V4), parameter DC= DC1/DC2 where DC2-density of color of intact B-cells (as 1.00) and DC1-B-cells in diabetes.