Abstract
Background. Growth tendency the asymptomatic forms of an urogenital trichomoniasis, frequency of complications from reproductive organs, uncertainty of many aspects of the violations of a spermatogenesis influencing reproductive function all this proves need of search of the urogenital trichomoniasis adequate experimental model. Lack of the corresponding experimental model is limited by our opportunities for carrying out the standardized, controlled researches on studying of transmission, pathogenesis, the immune answer, therapy and development of vaccines at a triсhomonas infection. Objective is studying action of Trichomonas vaginalis on a spermatogenny epithelium the mature of individuals of guinea pigs in the conditions of sharp and chronic experience. Materials and methods . Experiments are made on the “Reproductive System (Guinea Pigs) + Trichomonas vaginalis” modeling the natural course of an infection. In experiment 2 groups of animals: 1st (n = 8) – experimental, 2nd (n = 8) – control were formed. Against the background of the reduction of the immune status (hydrocortisone 125 mg/kg intramuscularly 1 time in day during 2 days) the animals of the 1st group were injected intraurethral suspension containing 1 × 106 Trichomonas on 0.5 ml of culture medium, the animals of the 2nd group – 0.5 ml of medium. Under the condition of the acute experiment the animals were sacrificed on day 9 (the middle of the cycle of spermatogenesis) – I experienced group and on day 30 (full spermatogenic cycle) – II experimental group. The control animals were slaughtered in the same period. The material for histological study was prepared by the traditional way. Results. In an experimental model of “Reproductive system (guinea pigs) + T. vaginalis”, staging and degree of disturbance of spermatogenesis, depending on the duration of trichomonas infection were shown. So, in acute experience morphological changes correspond to changes in the early inflammatory process; in chronic – a violation of stratification layers of seminiferous epithelium, degeneration of the cells, the appearance of binucleate cells. All of this indicate for abnormal cytokinesis with the possibility of blocking the formation of mature sperm.