The in vivo and in vitro diabetic wound healing effects of two wild plants growing in Kazakhstan and its mechanisms of action

TS Kustova, TA Karpenyuk, Н. А. Ибрагимова, CL Cantrell, Samir Anis Ross

2015

Closed access Metadata (CSL-JSON)
DOI 10.1055/s-0035-1565735
Data confidence 70 medium
How this score is calculated

It is built from checkable properties of the record itself: whether the article has a DOI, how many independent sources confirmed its data, whether the required fields are filled in, and how recent the latest confirmation is. Disagreement between sources and a missing identifier lower it. A value of 80 or above means the record was published without further checking; 60 to 79 means it is published at reduced confidence, with a manual check advisable. A record verified by an editor by hand is never pushed below 90 by a recalculation. This scores our data about the article, not the article itself.

Abstract

Diabetic complications, such as foot ulcer is a global health problem. Therefore, much attention has been paid to find biological active substances from plants that help to solve this problem. In our previous studies were shown antimicrobial and antioxidant properties of two plants growing in Kazakhstan ( Vexibia alopecuroides (L.) Jakovl. and Salvia deserta Shang). In this study they were examined for the ulcer healing effect in vivo , and its potential mechanisms of action in vitro . A streptozotocin induced diabetic foot ulcer rat model was used for studying the wound healing effect. The proliferative effects of crude extracts on MDCK cells were evaluated by the MTT assay. The migration of MDCK cells was examined using the scratch wound healing assay. Our in vivo results demonstrated a significant reduction of wound area at day 9 in both treated groups as compared to control group (p < 0.05). But only methylene chloride extract obtained from Vexibia alopecuroides could significantly stimulate proliferation in a dose dependent manner (p < 0.05). Besides, this extract could significantly increase the cell migration (p < 0.01). However, due to the complexity of a wound healing process in vivo , the methylene chloride extract obtained from Salvia deserta may still influence the healing through other pathways. Our study presents for the first time scientific evidence towards the efficacy of the two plants growing in Kazakhstan in enhancing diabetic wound healing.