RESEARCH OF ENZYMATIC HYDROLYSIS PROCESSES OF WHEAT'S STRAW BY LIQUID CELLULOLYTIC ENZYMATIC AGENTS

2018

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DOI 10.31224/osf.io/9hvdp
Data confidence 70 medium
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Abstract

Objective of this research was to investigate process of enzymatic hydrolysis of wheat's straw by liquid cellulolytic enzymatic preparations. At the first stage wheat straw has been subjected to autohydrolysis then the fibrous weight was washed out by water with reception of sugar solution. The following stage included extraction of disrupt lignin and modelling experiments for studying enzymatic kinetics was the last stage. As a result of the carried out researches it has been established, that that the maximum exit of reacting substances is observed 4,8 %, the minimum exit is 2,2 %. The general maintenance of carbohydrates in wheaten straw has made 83,3 % from weight of wheaten straw. Among monosaccharides in straw are prevailed xylose and glucose. Measurement of рН of the investigated wheaten straw in water at the hydromodule 1 : 5,8 has shown, that рН makes 7,2840±11. рН of straw presoaked in 0,59 % of weights to sulphurous acid has made 2,24±0,11. рН of the straw presoaked in 1,18 % of weights to sulphurous acid has made 1,48±0,10.