SCREENING OF ASPERGILLUSFUNGUS FOR CELLULASE BIOSYNTHESIS

2022

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DOI 10.53729/mv-as.2022.03.06
Data confidence 85 high
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Abstract

В статье приведены результаты исследования скрининга высокоактивного штамма Aspergillus niger 355 для дальнейшего использования в кормопроизводстве. Культуру выращивали в течение 45 суток при температуре 26-28°С на оптимизированной ранее питательной среде. Установлено, что после 6-9 суток культивирования наблюдается стабилизация биосинтеза фермента. Наибольшая целлюлазная активность наблюдалась на 9, 18 и 45 сутки культивирования. Активность целлюлазы варьировала от 1,63 до 3,5 ед/мл. При периодическом же культивировании Aspergillus niger 355 максимум образования фермента наблюдался на 3 сутки и составил 1.63 ед/мл с последующим падением ферментативной активности. The article presents the results of a study on the screening of a highly active strain of Aspergillus niger 355 for further application in feed production. The culture was grown for 45 days at a temperature of 26-28°C on a previously optimized nutrient medium. It was found that after 6-9 days of cultivation, stabilization in enzymes biosynthesis was observed. The highest cellulase activity has been observed on the 9th, 18th and 45th days of fungal cultivation. Enzymatic activity varied from 1.63 to 3.5 U/ml. In periodic cultivation of Aspergillus niger 355, the maximum enzymatic activity was observed on the 3rd day and was 1.63 U/ml, followed by a decrease in activity.