identification of genetic carriers of wheat steady againt common bunt Tilletia caries (DC.)

2019

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DOI 10.26577/eje.2019.v61.i4.06
Data confidence 70 medium
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Abstract

One of the most widespread and dangerous diseases of wheat is the common bunt, whichis caused by the fungi Tilletia caries (DC.). The most effective method of combating smut is consideredto be genetic protection of plants, which is achieved by the introduction of new resistant samples tocommon bunt wheat. There is information about more than 15 genes that can express resistance to thisdisease. A number of molecular markers associated with the main genes of resistance to common bunthave been developed. They will be used to create common bunt resistant samples by screening andintrogression of Bt-genes resistance into wheat samples with good economic value traits. The aim ofthe study is to identify carriers of resistance to common bunt using molecular markers. Molecular andphytopathological screening of Romanian wheat samples for resistance to common bunt Tilletia caries(DC.) was carried out. Molecular screening of Romanian samples for resistance to common bunt showedthat 2 samples (02429GP-1, F08245G1) possess the Bt9 gene. When using a primer for PCR to the FSD/ RSA locus, the Bt10 resistance gene was detected in two samples. As a result of phytopathologicalanalysis, it was found that 5 samples (02429GP-1, F08126G1, F08245G1, F08347G1, F07270G2) arehighly resistant to common bunt. Retezat showed a susceptible response with a lesion of 52%. The dataobtained are valuable in breeding programs to increase resistance to common bunt.Key words: wheat, molecular screening, resistance genes, phytopathological assessment, commonbunt.