Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots

Dmitriy V. Sotnikov, Lyubov V. Barshevskaya, Anatoly Vitalievich Zherdev, Saule Z. Eskendirova, К.К. Муканов, Kanatbek Mukantayev, Yerlan M. Ramankulov, Boris Borisovich Dzantiev

Applied Sciences, 2020

Full text Open access: Gold Metadata (CSL-JSON)

DOI 10.3390/app10030738

Data confidence 85 high

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Abstract

In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: Gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochromatographic serodiagnostics systems that use only one marker. The approbation of the test system was conducted on samples of cattle sera with low, but diagnostically significant titers of specific antibodies. We show that when two conjugates are used, the intensity of the detectable signal increases by 2–3 times compared with the test system using the QD conjugate and by more than nine times compared with the system using the GNP conjugate.