Abstract
As a consequence of development of combined method of purification and extraction of pectolytic enzyme, we have produced the enzyme with higher polygalacturonase (PGA) activity with a sufficiently high yield of the final product.The method is based on consecutive removal of individual groups of inactive impurities from enzyme solutions by means of activated charcoal and anion-exchange resin.Application of hydroxyapatite makes it possible to achieve activation of pectinases by removal of low molecular proteins.The absence of pectins and carbohydrates facilitates its usage in winemaking for clarification of superior wine grads and removal of wine stone composed of pectin substances of grape juice.