SELECTION OF HISTOPLASMA FARCIMINOSUM STRAIN TO CREATE A REMEDY FOR EQUINE LYMPHANGOITIS

2023

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DOI 10.52578/2305-9397-2023-4-1-138-149
Data confidence 70 medium
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Abstract

Epizootic lymphangitis is a chronic infectious disease, the causative agent of which is the fungus Histoplasma farciminosum and continues to cause significant outbreaks worldwide, despite several decades of epidemiological surveillance, diagnosis and prevention. Therefore, research on the selection of strains in the composition of anti-lymphangoitic agents is very relevant. The following strains of Histoplasma farciminosum fungus were used for selection: 410, T, YH, 5th, 4th, 5th, 17th, 21st, 8ZH. Microbiological studies of the studied strains were carried out with the study of cultural and morphological properties, the isolation of saccharolytic, proteolytic enzymes, the formation of catalase, oxidase, etc. To determine the biological properties of strains, agar and Saburo broth were used. In the course of the work, the cultural and biochemical properties of 9 strains of fungi were studied: 410, T, YH, 5th, 4th, 5th, 17th, 21st, 8ZH, as well as their growth on the Saburo nutrient medium. The validity of choosing a strain to create an anti-lymphangoitic agent of horses should include the following parameters: the strain, regardless of the type and genus, should have homogeneous cultural and morphological properties without signs of dissociation and abundant bacterial mass yield, In total, the selected strains will be a biological reserve that can be used in the development of diagnostic tools and prevention of equine lymphangoitis