Abstract
as you know, one of the economically and socially significant diseases that are widespread in the territory of the Republic of Kazakhstan is brucellosis, which occupies a dominant place in the general infectious pathology of animals. Brucellosis is one of the most dangerous zoonotic infectious diseases for humans. The results of comparative studies on the identification of the causative agent of brucellosis in the Republic of Kazakhstan for several years using the bacteriological method and PCR indicate that the use of the above methods to determine the epizootological status of herds of animals in the primary diagnosis of brucellosis is impractical due to the low degree of informativeness of these tests. PCR is recommended for identification and genotyping of isolated brucella cultures from pathological material. As a result of diagnostic studies of biomaterial obtained from animals from economically disadvantaged brucellosis subjects of Kazakhstan and border countries (Russia and Kyrgyzstan), the authors identified brucella cultures, which were subsequently subjected to the study of their biological and molecular genetic properties. For the identification and genotyping of isolated brucella cultures, researchers suggest using MLVA as the most effective method for reliably determining their genotypic characteristics.