Abstract
Abstract Objectives The majority of research programs had been focused on extract from the fruiting body. However, fungal mycelium obtained by submerged cultivation also has higher pharmacological potential. The aim of this study was investigation of the antioxidant and antimicrobial potential of Ganoderma lucidum and Trametes versicolor fungal mycelium biomass obtained by submerged cultivation and liquid fluid. Methods The antioxidant activity was evaluated by comparing the oxidation kinetics of the reduced form of 2,6-dichlorophenolindophenol with atmospheric oxygen in the presence/absence of biological samples. A criterion for evaluating for assessing the antioxidant activity was the values of the inhibition constant of the oxidation reaction. Antimicrobial activity of the fungal extracts was performed by agar disc diffusion method. Results A comparative analysis of antioxidant potential of G. lucidum and T. versicolor mycelium biomass and liquid fluid was conducted for the first time. It was found that antioxidant activity of fungal biomass depends on the cultivation time. The most active were the fractions obtained on the 21st day of fungal cultivation. Fungal extracts were more effective against Gram-positive bacteria compared to Gram-negative bacteria, micromycete and yeasts. Conclusions The results showed that submerged cultivation of mushrooms has significant industrial potential.