Micropropagation of rose cultivar Pareo

Mukhambetzhanov Serik, Nam Svetlana, Mursalieva Valentina, Rakhimbaev Izbasar

2011

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DOI 10.5897/ajb11.1301
Data confidence 70 medium
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Abstract

A protocol was developed for micropropagation of rose cv. ‘Pareo’. Nodal segments were surface sterilized with 0.1% solution of mercuric chloride for 10 min and these disinfected explants were inoculated aseptically on culture medium. The, axillary shoots were regenerated from nodal explants on agar-gelled Murashige and Skoog medium supplemented with 1.5 mg/l BAP. In vitro rooting was obtained when shoot clusters were cultured on half strength Murashige and Skoog medium supplemented with 1.0 mg/l IBA. The rooted plantlets were acclimatized successfully in the field. Key words: Micropropagation, nodal segments, rose.