2′‐OH of mRNA are critical for the binding of its codons at the 40S ribosomal P site but not at the mRNA entry site

D. M. Graifer, Andrey V. Zhigailov, Aliya G. Venyaminova, Alexey A. Malygin, Bulat K. Iskakov, Galina G. Karpova

2012

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DOI 10.1016/j.febslet.2012.09.003
Data confidence 85 high
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Abstract

The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capable of a tRNA(Phe)-dependent binding to human ribosomes and cross-linking to rpS26e in the mRNA binding centre. Thus, 2'-OH groups of mRNA are unimportant for binding at the entry site but they are crucial for codon-anticodon interactions at the P site, implying the existence of mRNA-ribosome contacts that do not occur in bacteria.