Screening of lipid degrading microorganisms for wastewater treatment

Zinigul Sarmurzina, Samat Kozhakhmetov, Sandugash Anuarbekova, Serik Shaikhin, K Kh Almagambetov

2013

Full text Open access: Gold Metadata (CSL-JSON)
DOI 10.21161/mjm.49612
Data confidence 70 medium
How this score is calculated

It is built from checkable properties of the record itself: whether the article has a DOI, how many independent sources confirmed its data, whether the required fields are filled in, and how recent the latest confirmation is. Disagreement between sources and a missing identifier lower it. A value of 80 or above means the record was published without further checking; 60 to 79 means it is published at reduced confidence, with a manual check advisable. A record verified by an editor by hand is never pushed below 90 by a recalculation. This scores our data about the article, not the article itself.

Abstract

Aims: Fats, oils and greases (FOG) are poorly removable materials in wastewater treatment systems.The aim of this work is to find the most suitable strain(s) for a biological treatment technology of FOGs polluted wastewaters. Methodology and results:The 142 microorganisms from polluted environment were screened for lipase activity (LA) by sequentially using assays on agar-Tween 80, agar-fats, and turbidimetrically measuring the quantity of calcium salts with fatty acids.The isolates G23, G30, and Zb32 showed highest units of LA and were identified by sequence analysis of 16S rRNA genes.Lipid masses were determined gravimetrically after chloroform/ethyl alcohol extraction.In the model solutions with animal fats the strain Pseudomonas aeruginosa G23 reduced mass fractions of mutton fat, beef tallow, and lard by 79±5%, 88±4%, and 80±6% respectively.Under the same conditions Aeromonas punctata G30 reduced: 65±3%, 60±8%, and 75±4 %, and P. aeruginosa Zb32 reduced: 47±5 %, 52±6% and 73±7%.In the model solutions with FOGs trap specimens as a carbon source from the local cafeteria the strains P. aeruginosa G23, A. punctata G30, and P. aeruginosa Zb32 reduced a lipid mass fraction by 61.5±7%, 45.2±5%, and 37.5±3% respectively.Conclusion, significance and impact of study: The strain P. aeruginosa G23 is the most effective lipid-degrading microorganism and the best candidate to use in biological treatment technology of FOGs polluted wastewater in Kazakhstan.