Abstract
spp. necessitates the development of resistance strategies that extend beyond conventional race-specific mechanisms. Although recent reviews (2023-2025) have emphasized gene discovery and genomic approaches, comparatively less attention has been given to the potential roles of metabolic regulation and micronutrient homeostasis in host-pathogen interactions. Here, we present a narrative synthesis of current evidence and propose a conceptual framework in which induced mutagenesis (ethyl methanesulfonate, EMS, and γ-irradiation) serves as a tool for investigating interactions among redox regulation, iron (Fe) homeostasis, and disease resistance. A key component of this framework is the proposed interplay between reactive oxygen species (ROS) signaling and Fe partitioning. Vacuolar iron transporters (VITs), ferritins, and associated transport networks regulate intracellular Fe distribution and may influence Fe availability at the host-pathogen interface, potentially affecting fungal development and host defense responses. This concept of "iron-withholding immunity" may operate alongside ROS-mediated defense processes, linking metabolism with immune function. Observations from mutant wheat populations are broadly consistent with the hypothesis that these processes may contribute to durable adult-plant resistance (APR), which is characterized by reduced disease development, coordinated defense responses, and relative stability across environments. In some studies, Fe-enriched mutant lines have been associated with enhanced expression of pathogenesis-related genes and the occurrence of combined APR and seedling-resistance phenotypes, suggesting possible links between micronutrient homeostasis and immunity. Integration of high-throughput phenotyping with genotype × environment × time (G × E × T) frameworks may further improve our understanding of quantitative resistance and disease-associated traits. Overall, this review highlights the potential importance of nutrient homeostasis, redox regulation, and susceptibility modulation as components of future research aimed at developing climate-resilient and nutritionally improved wheat cultivars.