Comparative Histochemical Analysis of the Aerial Parts of Fragaria viridis and Fragaria vesca (Rosaceae) Growing in the Central Kazakhstan Region

Gulnissa K. Kurmantayeva, Ishmuratova M. Yu., Gayane A. Atazhanova, Samorodov A.V., Мarlen К. Smagulov, Aliya Amangeldievna Ramazanova

2025

Closed access Metadata (CSL-JSON)
DOI 10.52711/0974-360x.2025.00696
Data confidence 70 medium
How this score is calculated

It is built from checkable properties of the record itself: whether the article has a DOI, how many independent sources confirmed its data, whether the required fields are filled in, and how recent the latest confirmation is. Disagreement between sources and a missing identifier lower it. A value of 80 or above means the record was published without further checking; 60 to 79 means it is published at reduced confidence, with a manual check advisable. A record verified by an editor by hand is never pushed below 90 by a recalculation. This scores our data about the article, not the article itself.

Abstract

Histochemical Analysis of Medicinal Plants and Raw Materials is a method aimed at studying the localization of chemical substances and their metabolic products in plant tissues. This article presents the results of the histochemical analysis of the leaves and petioles of "Fragaria viridis" and "Fragaria vesca". The genus "Fragaria" belongs to the Rosaceae family, and includes several species known for their high content of biologically active compounds, including phenolic compounds, flavonoids, and lipids. These substances contribute to the medicinal properties of strawberries, making them valuable both in traditional medicine and modern pharmacology. Objective: To determine the characteristics of the localization of specific groups of secondary metabolites in the aerial parts of "Fragaria viridis" and "Fragaria vesca" growing in the Central Kazakhstan region at the microscopic level. Materials and Methods: Transverse sections of the leaves and petioles of F.viridis and F.vesca were fixed in Strauss-Fleming solution, after which histochemical reactions were performed to determine the location and identify essentialoils, phenolic acids, flavonoids, sesquiterpene lactones, polysaccharides, and alkaloids. The study of microscopic features and histochemical tests was conducted according to the methods of the State Pharmacopoeia of the Republic of Kazakhstan using a Biomed-4 light microscope. Results: Histochemical methods using light microscopy were employed to determine the localization of biologically active substances in the studied plant raw material. The presence of phenolic acids, flavonoids, and essential oils was detected. The absence of sesquiterpene lactones, polysaccharides, and alkaloids in the examined material was established. Conclusion: For the first time, histochemical tests were used to study the localization of secondary metabolites in the tissues of "F. viridis" and "F. vesca". The results of these histochemical studies can be utilized for confirming authenticity, identification, and standardization of the aerial parts of "F. viridis" and "F. vesca".