Abstract
The article presents methods of express diagnostics and a laboratory method for determining the presence of ketone bodies in blood and urine. The research was carried out in 2018-2019 at the Department of "Veterinary Medicine" of NJSC «Kazakh Agrotechnical University named after S. Seifullin», in the farms of Akmola, Pavlodar and North Kazakhstan regions. The objects of research are cows of Holstein black-and-white and Simmental breeds (n=60) of the first and second lactation, during the early and late start, as well as in the first two weeks after calving. All breeding stock, taking into account the level of ketone bodies, was divided into groups: up to 1 mmol/l – clinically healthy, 1-2,5 mmol/l – subclinical ketosis, more than 2,5 mmol/l – clinically pronounced ketosis. Diagnosis of ketosis and diseases of reproductive function was carried out taking into account the early and late start, as well as the timing after calving cows. Ketone bodies in urine were determined in a qualitative and semi-quantitative way by the equipment of Keto-txt and Girul, Dirul Industrial Co., LTD and according to the Lestrade test method in laboratory conditions. The effectiveness of diagnostic methods for clinical and subclinical ketosis is 85-90%, the laboratory method of urine according to Lestrade is 87%, ultrasound examination of the reproductive system is 80%, rectal examination is 85-93%.