Abstract
Bifidobacterium pullorum, commonly found in chicken waste and preferring mesophilic characteristics, contains an enzyme known as Beta-Xylanase. This enzyme effectively breaks down xylan, offering potential for creating biogas, like methane, and biofuels, such as ethanol. Scientists are actively exploring sustainable energy sources, while industries aim for cost-effective methods to decrease operational expenses. The conventional methods for producing biogas and biofuels involve high-temperature processes using fuel combustion, leading to significant expenses. To address this, mesophilic bacteria present a promising alternative for more cost-efficient biofuel production. This study is the first to delve into the genomic and three-dimensional structure of beta-xylanase, crucial for breaking down xylan. Our findings highlight that the beta-xylanase in Bifidobacterium pullorum showcases a TIM-barrel structure, similar to other GH10 xylanases essential in carbohydrate breakdown. This indicates a potential connection between Bifidobacterium pullorum's beta-xylanase and the improvement of biogas production.