Genetic Diversity of Inbred Sunflower Lines of the Kazakhstan Collection Fund for Protein and SSR Markers

Kulpash Bulatova, Shynar Mazkirat, O. A. Gavrilova, D. A. Yusaeva, D. I. Babissekova, Perizat Alchinbayeva

2020

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DOI 10.3103/s009545272001003x
Data confidence 70 medium
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Abstract

Abstract This paper presents results of studying the genetic diversity of 126 sunflower inbred lines from the collection fund of the Experimental Farm of Oilseeds of Kazakhstan for protein and SSR markers. The polymorphism of collections in terms of the composition of helianthinin, seed storage protein, was not high. In the zone of fast-moving proteins, three variants of components were found that are characteristic of all types of inbred lines. At the same time, components specific to lines with genes rf and Rf were identified. PCR analysis of DNA of inbred lines by 16 SSR markers showed no variations in the amplification products of locus ORS428 and low polymorphism of locus ORS785 (PIC ≤ 0.2). The following markers in the studied collections showed high polymorphism (PIC > 0.8): ORS316, ORS381, ORS437, ORS456, ORS505, ORS630, ORS761, and ORS925. Based on molecular marking, the registration of inbred lines of the Kazakhstan Collection Fund was carried out. In order to assess the level of hybridity of F1 seeds of commercial sunflower hybrids, SSR markers were selected that can differentiate the genetic material of the original parental lines in the DNA of the hybrid.