Pigment mutants of the green microalga Chlamydomonas reinhardtii: Morphological properties and photosynthetic performance

Asemgul K. Sadvakasova, Nurziya R. Akmukhanova, Bolatkhan K. Zayadan, Dmitry N. Matorin, Ф. Ф. Протопопов, А. А. Алексеев, Kenzhegul Bolatkhan

2016

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DOI 10.1134/s1021443716040130

Data confidence 85 high

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Abstract

Pigment mutants CC-124y-1, СС-124у-2, and СС-124р-2 of the green microalga Chlamydomonas reinhardtii Dangeard were obtained by means of UV-induced mutagenesis. Morphological properties and photosynthetic activity of these mutants were examined. The mutants displayed a lowered content of chlorophyll b and carotenoids. Analysis of fluorescence induction curves revealed the decreased rates of electron transport in photosystem II (PSII), the increase in the fraction of Q B -nonreducing centers, and the enhancement of nonphotochemical fluorescence quenching. The mutations had no direct impact on oxidation of pigment P 700 in PSI and on the decline of delayed fluorescence. The parameters of induction curves of prompt and delayed fluorescence are proposed for use in early diagnostics of UV-induced mutagenesis. The pigment mutants obtained can be applied in biomonitoring studies.