Abstract
A high-fat, high-sugar diet (HFHS) is known to exacerbate oxidative stress and behavioral dysfunctions, increasing susceptibility to cognitive decline. This study aimed to evaluate the effects of Stevia rebaudiana and Stachys sieboldii on lipid peroxidation and behavioral alterations in rats fed an HFHS diet. Forty Wistar rats were divided into four groups: a control group on a standard diet, an HFHS group, and two experimental groups receiving HFHS supplemented with either Stevia or Stachys for 30 days. Behavioral responses were assessed using the Open Field Test, and oxidative stress markers (malondialdehyde, conjugated dienes, ketodienes, and Schiff bases) were measured in brain homogenates. Stevia significantly reduced oxidative stress markers by 30–51%, whereas Stachys decreased hyperactive locomotor behavior by 63–68%. Correlation analysis revealed strong associations between lipid peroxidation and behavioral parameters. These findings suggest that Stevia is more effective in reducing oxidative stress, while Stachys better regulates excessive locomotion. Both plant-based supplements exhibit neuroprotective potential, with distinct mechanisms of action, supporting their use as dietary interventions to mitigate the effects of an HFHS diet on brain function.