Abstract
The article presents the results of a histochemical study of the aerial part of Linaria vulgaris Mill., collected during the period of mass flowering (July-August 2023) in the Bukhar-Zhyrau district, Karaganda region (Republic of Kazakhstan). Goal. To ascertain the specific localization patterns of various groups of biologically active substances within the aerial organs of Linaria vulgaris Mill., as found in the Central Kazakhstan region. Materials and methods. For the histochemical analysis, aerial parts of Linaria vulgaris Mill. were collected during the flowering stage. These plant samples were fixed in Strauss-Fleming solution, followed by preparation of cross sections. Histochemical reactions were then carried out to pinpoint and characterize various biologically active compounds, including essential oils, starch, flavonoids, polysaccharides, sesquiterpene lactones, and alkaloids. The microscopic examination and histochemical tests adhered to the protocols outlined in the State Pharmacopoeia of the Republic of Kazakhstan. Results. Histochemical techniques employing light microscopy were used to identify the distribution of biologically active compounds within the plant raw material of Linaria vulgaris Mill. The analysis confirmed the presence of flavonoids, phenolic compounds, alkaloids, and polysaccharides. However, essential oils and sesquiterpene lactones were not observed. Conclusion. This marks the inaugural application of histochemical tests to examine the distribution of biologically active substances within the tissues of Linaria vulgaris Mill. These findings hold significant potential for verifying the authenticity, identifying, and establishing standards for the aerial components of Linaria vulgaris Mill.