Abstract
Abstract— Applicability of three strains of green microalgae (Chlorella vulgaris SP BB-2, Parachlorella kessleri, and Chlamydomonas reinhardtii Dangeard СС-124) for bioremediation (contaminants removal) of aquaculture wastewater was investigated. C. vulgaris strain SP BB-2 exhibited the highest growth rate and biomass accumulation. This strain was found to utilize nitrogen and phosphorus from wastewater for growth and development and showed high efficiency according to chemical oxygen demand (COD). Moreover, among the studied strains, C. vulgaris SP BB-2 produced biomass with the highest protein content, which may be recommended as a feed additive for the aquaculture. Analysis of the induction and rapid light curves of chlorophyll fluorescence revealed alterations in the energy storage photosynthetic processes of C. vulgaris SP BB-2 cells when grown on the aquaculture wastewater. Thus, high values of the maximum quantum yield of primary photochemistry in photosystem II (PSII) (FV/FM), quantum yield of electron transport (φEo), PSII performance index on absorption basis (PIABS), and the light utilization coefficient (α) were observed. C. vulgaris strain SP BB-2 may be recommended for wastewater treatment, while its highly sensitive parameters of chlorophyll fluorescence induction (PIABS, φEo) may be used as indicators of the state of the microalgae.