Creation of a heterologous gene expression system on the basis of Aspergillus awamori recombinant strain

А. М. Рожкова, Анна Сергеевна Середа, Нина Васильевна Цурикова, A. K. Nurtaeva, Margarita V. Semenova, Любовь Вячеславовна Римарева, Ekaterina A. Rubtsova, Ivan N. Zorov, Olga A. Sinitsyna, Arkady Panteleimonovich Sinitsyn

2011

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DOI 10.1134/s0003683811030124
Data confidence 70 medium
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Abstract

A heterologous gene expression system was created in a domestic Aspergillus awamori Co-6804 strain, which is a producer of the glucoamylase gene. Vector pGa was prepared using promoter and terminator areas of the glucoamylase gene, and A. niger phytase, Trichoderma reesei endoglucanase, and Penicillium canescens xylanase genes were then cloned into pGa vector. Separation of enzyme samples using FPLC showed the amount of the recombinant proteins to be within the 0.6-14% range of total protein.