Isolation of Central Asian Subspecies of Tularemia Agent in the Altai Territory

Alexander N. Mokrievich, Vitalii S. Timofeev, T. Yu. Kudryavtseva, G. I. Ulanova, S. B. Karbysheva, Миронова Раиса Ивановна, Г. М. Вахрамеева, T.I. Gubareva, В. М. Павлов, Ivan Alekseevich Dyatlov

Problems of Particularly Dangerous Infections, 2013

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DOI 10.21055/0370-1069-2013-1-66-69

Data confidence 70 medium

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Abstract

Within the frames of activities attributed to the Reference Center for tularemia monitoring at SRC AMB, genetically identified are 4 isolates of Francisella tularensis , isolated in 2011 in the Altai Territory. These bacteria prove to be virulent for BALB/c mice, DCL being lower than 10 CFU. Using single-primer PCR-typing and MLVA assay distinguished have been the subspecies of the isolates. Three of them refer to the Central Asian subspecies, one – to the Holarctic, the former being isolated in the territory of the Russian Federation for the first time ever.