3D structured capillary cell suspensions aided by aqueous two-phase systems

Amro K. F. Dyab, Vesselin N. Paunov

2024

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DOI 10.1039/d4tb01296h
Data confidence 85 high
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Abstract

We report a facile technique for 3D structuring of living cells by forming capillary cell suspensions based on an aqueous two-phase system (ATPS) of polyethylene glycol (PEG) and dextran (DEX) solutions. We demonstrate the formation of water-in-water (DEX-in-PEG) capillary bridges using concentrated suspensions of yeast cells which show enhanced rheological properties and distinctive 3D patterns. Capillary structured cell suspensions can potentially find applications in novel ways of 3D cell culturing, instant tissue engineering and many biomedical investigations.