Abstract
This study focuses on the phytochemical composition and different biological activities of A setacea Waldst.et Kit, a wild species indigenous to the Ortau Mountain in the Karaganda region of the Republic of Kazakhstan.Utilizing the ultrasoundassisted extraction method, we evaluated the extraction efficiency through phytochemical analysis.Phenolic compounds in the extract were identified and quantified using a high-performance liquid chromatograph coupled with a diode array detector (HPLC-DAD).The major phenolic compounds identified in the methanol extracts of Ach.setacea were chlorogenic acids, coumarins, ellagic acids and apigenin in flowers (17.48, 13.84, 7.87 and 55.47 µg/g, respectively), aerial parts (73.41, 18.20 and 10.25, 3.76 µg/g, respectively) and roots (14.79, 9.95 and 4.02 µg/g, respectively).In comparison with the underground portion of the plant extracts, flowers and aerial parts exhibited higher total phenolic and flavonoid contents.For instance, chlorogenic acid, coumarin, myricetin, quercetin, apigenin, and ellagic acid show higher amounts in the flower and the plant's aerial part than the underground part.Furthermore, we found that hexane-based root extracts demonstrated superior cholinesterase and diabetic inhibitory activities compared to methanol extracts.On the contrary, methanol extracts exhibited higher uretic and tyrosinase inhibitory activities compared to those obtained from hexane extract.