Abstract
In this article, the biocatalytic oxidation of ethanol into acetaldehyde was studied using a catalase entrapped within a monolithic polyampholyte cryogel, p(APTAC-co-AMPS), as catalyst. When an anionic monomer, 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS), was mixed with a cationic monomer, (3-acrylamidopropyl) trimethylammonium chloride (APTAC), under cryo-polymerization conditions at a molar ratio of monomers [APTAC]:[AMPS] = 75:25 mol.% in the presence of 10 mol.% cross-linking agent, N,N-methylenebisacrylamide (MBAA), the macroporous polyampholyte cryogels containing various amounts of catalase were synthesized in situ. The conversion of ethanol into acetaldehyde in good-to-high yields was observed in flow-through and batch-type reactors under optimal conditions: at T = 10–20 °C, pH = 6.9–7.1, [C2H5OH]:[H2O2] = 50:50 vol.%. According to the SEM images, the pore sizes of the p(AMPS-co-APTAC) cryogel vary from 15 to 55 μm. The catalytic activity of catalase entrapped within a monolithic polyampholyte cryogel in the conversion of ethanol into acetaldehyde was evaluated through the determination of kinetic parameters such as the Michaelis constant (Km), maximum enzymatic rate (Vmax), activation energy (Ea), turnover number (TON) and turnover frequency (TOF). The catalase encapsulated within the monolithic polyampholyte cryogel exhibits a high conversion of ethanol into acetaldehyde. The key parameters of ethanol oxidation in flow and batch reactors in the presence of the cryogel monolith were calculated.