Development of Spectral Imaging Cytometry

Ivan A. Vorobjev, Aigul Kussanova, Natasha S. Barteneva

2023

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DOI 10.1007/978-1-0716-3020-4_1
Data confidence 70 medium
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Abstract

Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry.