Abstract
An endo-β-1,4-glucanase (EG), produced under submerged fermentation by local isolate Trichoderma atroviride, was purified using ammonium sulfate precipitation, column and ion exchange chromatography with 55.16 fold and a specific activity of 30.9 EU mg -1 .The studies of PAGE, SDS-PAGE and zymogram test have been carried out.The EG had optimum activity at pH 5.0 and 50 °C respectively.Using CMC as substrate, the enzyme showed maximum activity (V max ) of 6.7 (µmol glucose min -1 ) mL -1 with its corresponding K m (Michaelis-Menten constant) value of 1.12 mg mL -1 .While the EG activity was activated by NaCl, inhibited by MgCl 2 and MgSO 4 .Otherwise, Tween 80, Triton X-100 and the total saponins known as biosurfactants enhanced the EG activity.The obtained EG had low K m and high thermal stability.Additionally, usability of the EG in biotechnological application was investigated.The results showed that the EG had potentially sufficient effects on denim fabric and pretreated lignocellulosic wastes.