Abstract
The article presents a preliminary GC-MS profile and antifungal activity of a hydroethanol extract of Chelidonium majus L. collected in the high-altitude conditions of the Kungei Alatau range (Kazakhstan). Antifungal activity was evaluated in vitro against one Fusarium spp. isolated from potato tubers with symptoms of fusarium lesion. The extracts were obtained by Soxhlet extraction using 70% ethanol. The mycelial growth area was determined from digital images using ImageJ software. The experiment was carried out in four repetitions (n = 4). In the treatment with hydroethanolic extract of C. majus No. 1, the mycelial growth area of Fusarium spp. was 60.25 ± 4.08%, compared with 72.50 ± 2.65% in the solvent control containing 70% ethanol. Inhibition of mycelial growth relative to the solvent control was 16.9%. GC-MS analysis tentatively identified 11 compounds in the extract, among which ethyl esters of unsaturated fatty acids accounted for a substantial proportion (43.4%), followed by phytol (13.8%) and the compound 4H-Bis[1,3]benzodioxolo[5,6-a:4’,5’-g]quinolizine, 6,7,12b,13-tetrahydro-, (±) - (30.2%), tentatively identified based on mass-spectral library matching. The results obtained indicate the inhibitory activity of the hydroethanol extract of C. majus against the mycelial growth of Fusarium spp. in vitro and justify the need for further research.