Abstract
The natural originality of medicinal plants determines the particularities of their use in modern medicine. Herbs with a wide ecological range are well established and widely used in the pharmacological industry. The medicinal characteristics of Ferula asafoetida L. have long been known in Central Asian countries. But the scientific approach to studying the phenomenal plant began to take hold relatively recently. The aim of this work was to carry out a phytochemical analysis and study the pharmacological properties of Ferula asafoetida extract obtained from self-picked raw materials and to study the possibility of its use for wound healing. To observe the effectiveness of the resulting methanol extract, two series of experiments were set up to simulate wounds in rats. Series A evaluated the efficacy of a 4% Vaseline ointment based on the extract obtained in the treatment of wounds. Centella asiatica extract was used as a reference drug. In series B, a fibroblast culture was transplanted to the wound surface. 48 hours before transplantation, the cells were treated with herbal preparations. According to the MTT test, Ferula asafoetida L. extract significantly increased fibroblast viability in vitro compared to Centella asiatica. In a staged study of wound healing, no significant differences were observed between the groups when herbal extract ointments were used. When fibroblast cultures treated with herbal preparations were transplanted, faster wound surface closure was recorded on day 7 and 14 compared to untreated cell culture. But no visible advantage was noted in the use of Ferula asafoetida L. extract when compared with Centella asiatica. This may be due to the lack of antiradical activity of the extract under study. At the same time, significant antimicrobial activity of preparations of Ferula asafoetida L. compared to Centella asiatica has been found. Based on the data obtained, it was concluded that further search for ways to improve the antioxidant properties of Ferula asafoetida L. extract to enhance its effectiveness as a fibroblast stimulation agent with concomitant antimicrobial action is necessary.