LATEX ANTIGEN FOR SEROLOGIC DIAGNOSIS OF EMPHYSEMATOUS CARBUNCLE IN ANIMALS

2026

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DOI 10.52081/bkaku.2026.v77.i2.341
Data confidence 85 high
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Abstract

Studies in latex agglutination reaction (LAR) using Clostridium chauvoei antigen showed the specificity and suitability of the method for the detection of specific antibodies in animals vaccinated against this disease and suspected to have emphysematous carbuncle disease. It was found that a serum LAR titer of 1:30 -1:40 in immunized animals 30 days after vaccination can be considered as sufficient, and titers of 1:80 and 1:160 as a high level of immune response. When studying the dynamics of postvaccinal antibody titers in immunized animals under production conditions, it was found that the average antibody titer in LAR 30 days after vaccination was equal to 1:33. In animals immunized against emkar the sufficient level of antibodies was preserved only up to 90 days, on day 150 the average titers of postvaccinal antibodies decreased twice in comparison with titers established on day 30, and in 180 days in 4 times. It should be noted that in studies 180 days after vaccination, antibodies were not detected in 30% of animals. The results of the conducted studies should be taken into account when organizing and conducting prophylactic immunization of animals against emphysematous carbuncle.