Abstract
Yellow rust (Puccinia striiformis f. sp. tritici) is one of the most destructive diseases in wheat (Triticum aestivum L.) in Kazakhstan, causing significant yield losses. Owing to the high susceptibility of widely cultivated varieties, the development of resistant genotypes remains a key objective for sustainable crop protection. The aim of this study was to evaluate the resistance of wheat lines to yellow rust and to identify effective resistance genes. The research was conducted under artificial infection conditions using hybrid populations of the F2–F5 generations. The genotypes were assessed and ranked according to their resistance levels, and molecular markers were applied to detect resistance genes. Significant variability in disease response was observed. Analysis of variance revealed a strong effect of genotype on the infection coefficient (p < 0.001). Lines from later generations (F5) presented lower infection levels. Most genotypes carried the Yr5 gene, highlighting its major role in resistance, whereas Yr10 was less common. Yr15 and Yr18 were detected in some lines and were associated with partial (adult plant) resistance. Moderately susceptible forms predominated, indicating widespread quantitative resistance. However, highly resistant lines (CI = 0–1) and immune forms were identified, representing valuable material for breeding programs.