Abstract
The widespread use of antibacterial agents in livestock production is associated with antibiotic residues in animal products and feed, posing a potential threat to food safety and contributing to antimicrobial resistance. The aim of this study was to assess contamination levels of beef, pork, and feed with antibiotic residues in the Kostanay region. The results demonstrated the presence of antibiotic residues in beef, pork, and feed, while all detected concentrations remained below established maximum residue limits. Despite compliance with regulatory standards, residues were detected frequently, and their levels differed significantly depending on feed type (p < 0.001). Microbiological analysis confirmed the presence of Escherichia coli and Staphylococcus aureus in meat samples, with isolates exhibiting moderate to high antimicrobial resistance, particularly in pork. The highest resistance was observed to tetracycline, streptomycin, and thiamphenicol. The obtained data indicate that even trace antibiotic concentrations entering the “feed–animal–product” chain may be associated with selective pressure on microbiota and the circulation of resistant microorganisms. The results highlight the need for comprehensive monitoring of feed and animal products to assess and mitigate antimicrobial resistance spread within the food chain.