Modification of plant genomes using DNA technologies: progress in the delivery of exogenous DNA

2026

Full text Open access: Gold Metadata (CSL-JSON)
DOI 10.32523/2616-7034-2026-154-1-77-92
Data confidence 70 medium
How this score is calculated

It is built from checkable properties of the record itself: whether the article has a DOI, how many independent sources confirmed its data, whether the required fields are filled in, and how recent the latest confirmation is. Disagreement between sources and a missing identifier lower it. A value of 80 or above means the record was published without further checking; 60 to 79 means it is published at reduced confidence, with a manual check advisable. A record verified by an editor by hand is never pushed below 90 by a recalculation. This scores our data about the article, not the article itself.

Abstract

The article reviews data on the delivery of exogenous DNA fragments used in DNA technologies as expression cassettes for heterologous genes in the creation of transgenic plants, or as tools for modifying plant genomes using genome editing methods. The main focus is on in planta delivery methods that allow researchers to bypass the plant cultivation stage, which includes the disintegration of plant tissues into callus cultures followed by the in vitro regeneration of plant regenerants. It is this stage of plant genome modification using modern DNA technologies that is currently attracting the attention of many research groups. Data are presented on the prospects of using viral vectors for the transient delivery of genome editing tools into plant cells. The possibilities and some disadvantages of this technology are discussed, as well as the prospects for its application to agricultural plant species.