Abstract
This study evaluated the agronomic and qualitative characteristics and cold resistance of 46 doubled haploid mutant B. napus lines (M2 and M3) that were obtained by treating haploid embryos derived from a culture of isolated microspores with the EMS mutagen (8 and 12 mM).The fatty acid composition of seed oil, the agronomic characteristics forming the yield and the resistance to cold temperatures were considered.The analyses showed highly significant differences between the mutants and original cultivars in all the studied characteristics.Mutant lines DHK12-3, DHK12-4, DHK12-8, DHG12-16, DHG12-18 and DHG12-10 combined improved indicators of fatty acid composition with high yield and cold-tolerance.Only mutant lines obtained at a high mutagen concentration (12 mM) were more resistant to the cold than the original cultivars.The obtained lines were homozygous since they were doubled haploids, and the stability of agronomic and qualitative traits was observed in two generations.The results obtained confirm the higher productivity of doubled haploid mutant lines, as well as their availability to variable environmental conditions.