Abstract
This article presents a genetic analysis of the species identification of the natural microflora composition of brown coal waste from the Lenger deposit in the Turkestan region and a PCR analysis of pure microorganism strains. Understanding the ecological significance of microorganisms in brown coal waste allows us to assess their impact on the environment, agriculture and energy production processes. It has been established that the microflora of brown coal waste includes cellulose-destroying and heterotrophic bacteria, micromycetes and actinomycetes. The main species composition was represented by micromycetes from the genera Mucorales, Aspergillus, Fusarium, Candida, and bacteria from the genera Bacillus, Rodococcus, Pseudomonas, as well as nitrogen-fixing bacteria. As a result of molecular genetic analysis to identify bacterial strains using the Sanger sequencing method, the following were identified: LB.1 strain – Priestia megaterium, LA.8 strain – Pseudomonas sp., LB.M2 strain – Aspergillus sydowii. This research allows to deeply understand the role of beneficial microorganisms in industrial wastes of brown coal in ecological processes, their significance in nature and in production. The obtained research results help to determine the way of effective use of microorganisms in brown coal waste to increase soil fertility or use for biotechnological purposes.