IDENTIFICATION OF COXIELLA BURNETII USING SEQUENCING AND PHYLOGENETIC ANALYSIS OF THE 16S RRNA GENE

Абаева Мадина Рахметуллакызы, A.U. Isabek, Aibarys M. Melisbek, Arailym Bopi, Шыныбекова Гаухар Орынбековна, Nurlan Kozhabergenov, Meirim D. Almezhanova, Kulyaisan T. Sultankulova

2024

Full text Open access: Gold Metadata (CSL-JSON)

DOI 10.25205/978-5-4437-1691-6-216

Data confidence 70 medium

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Abstract

The 16S rRNA gene carries both conservative and variable regions of the nucleotide sequence, which allows it to be used for both genus and species typing of microorganisms. Using the sequencing method and phylogenetic analysis of the 16S rRNA gene, Coxiella burnetii was identified in the body of a tick from the Turkestan region of the Republic of Kazakhstan. The results of the phylogenetic analysis of the 16S rRNA gene showed that the causative agent of Q fever Coxiella burnetii was present in the sample isolated from a tick from the Turkestan region in 2023. Molecular typing of the Coxiella burnetii isolate revealed the MST 6 type.